Journal: Immune Network

Article Title: Effects of IL-3 and SCF on Histamine Production Kinetics and Cell Phenotype in Rat Bone Marrow-derived Mast Cells

doi: 10.4110/in.2010.10.1.15

Figure Lengend Snippet: TEM of rat bone marrow cells. Rat bone marrow cells cultured in the presence of rrIL-3 and rmSCF were analyzed by TEM at day 0 (A), day 4 (B), day 11 (C), and day 15 (D).

Article Snippet: Both rrIL-3 and rmSCF were purchased from Biosource International-Invitrogen (Camarillo CA, USA) or from R&D Systems (Minneapolis MN, USA) ( , ).

Techniques: Cell Culture

Journal: Immune Network

Article Title: Effects of IL-3 and SCF on Histamine Production Kinetics and Cell Phenotype in Rat Bone Marrow-derived Mast Cells

doi: 10.4110/in.2010.10.1.15

Figure Lengend Snippet: Cell proliferation, RMCP II production, and histamine synthesis in rat bone marrow cells treated with rrIL-3 and rmSCF. Rat BM cells were incubated under specified culture condition. Cells numbers were counted using a hemocytometer (A~C). RMCP II expression was analyzed with 200 ng/ml cell lysate of cultured rat bome marrow cells (D~F). Histamine levels were analyzed using a Histamine EIA kit (G~I). Culture conditions were controlled in in terms of serum (FBS vs. HS), media (IMDM vs. RPMI) or percoll concentration (50% vs. 70%). Open/white symbol or bar, FBS; and closed/gray symbol or bar, HS. In the line graph: circle, cells separated by 50% percoll; and triangle, cells by 70% percoll. Solid line, IMDM; and dash-dot line, RPMI-1640. : 50 IM FBS, : 50 IM HS, : 50 RP HS, : 50 RP HS, : 70 IM FBS, : 70 IM HS, : 70 RP FBS, : 70 RP HS. In the bar graph, bar with horizontal line, 50% percoll and IMDM; bar with oblique line, 50% percoll and RPMI-1640; bar with vertical line, 70% percoll and IMDM; bar with net cross, 70% percoll and RPMI-1640. : 50 IM FBS, : 50 IM HS, : 50 RP FBS, : 50 RP HS, : 70 IM FBS, : 70 IM HS, : 70 RP FBS, : 70 RP HS. IM for IMDM, RP for RPMI-1640, NA for not applied, ND for not determined. Y axis for both H and I in log scale.

Article Snippet: Both rrIL-3 and rmSCF were purchased from Biosource International-Invitrogen (Camarillo CA, USA) or from R&D Systems (Minneapolis MN, USA) ( , ).

Techniques: Incubation, Expressing, Cell Culture, Concentration Assay

Journal: Immune Network

Article Title: Effects of IL-3 and SCF on Histamine Production Kinetics and Cell Phenotype in Rat Bone Marrow-derived Mast Cells

doi: 10.4110/in.2010.10.1.15

Figure Lengend Snippet: The effects of cytokine treatment on rat bone marrow cell proliferation and differentiation. The specified cytokines were added to activeted rat bone marrow cells at culture day 8, which were incubated for 3 (black bar) or 7 (white bar) days more. At the indicated time point, cell proliferation was analyzed CCK-8 (A and C) and cell differentiation was analyzed by RMCP II production (B and D). Cells harvested on a 70% Percoll cushion (A and B). Cells harvested on a 50% Percoll cushion. (C and D). rrIL-3, recombinant rat interleukin 3; rmSCF, recombinant rat stem cell factor; RMCP II, rat mast cell protease II; rrIL-4, recombinant rat interleukin-4; rrIL-6, recombinant rat interleukn-6; rmIL-3, recombinant mouse interleukin-3; LPS, lipopolysaccharide; Con A, concanavalin A. Cell number is represented as times of the given condition described in material and methods.

Article Snippet: Both rrIL-3 and rmSCF were purchased from Biosource International-Invitrogen (Camarillo CA, USA) or from R&D Systems (Minneapolis MN, USA) ( , ).

Techniques: Incubation, CCK-8 Assay, Cell Differentiation, Recombinant

Journal: PLoS ONE

Article Title: Rat Embryonic Mast Cells Originate in the AGM

doi: 10.1371/journal.pone.0057862

Figure Lengend Snippet: (A) After 21 days in culture with SCF and IL-3 the MCcp grew as small colonies around the immunomagnetic beads (white arrows). (B) After 21 days in culture, many of the mast cells displayed metachromatic granules. Inset: A higher magnification of a metachromatic mast cell attached to a immunomagnetic bead. (Immunomagnetic beads, black arrows).

Article Snippet: The isolated AA4−/BGD6+ mast cells were cultured, (10 6 cells/ml), in Iscove's Medium supplemented with penicillin (100 U/ml), streptomycin (100 µg/ml), amphotericin B (0.25 µg/ml; Invitrogen, Gibco), 0.05 mM β-mercaptoethanol, 10% fetal calf serum, 100 ng/ml recombinant stem cell factor (SCF) (Invitrogen, Biosource) and 20 ng/ml recombinant interleukin-3 (IL3) (Invitrogen, Biosource).

Techniques: